Fig. 4

Steroid hormone receptor binding differs between male breast cancer subtypes. a Unsupervised hierarchical clustering of RNA-seq of 46 male breast tumors, using the M1/M2 classifier genes defined by Johansson et al.⁴ Standardized gene expression value is denoted as the row Z-score and plotted in high expression (blue) and low expression (yellow) scale. Top row indicates M1 (red) or M2 (blue) classification. b Correlation plots of significantly differentially bound regions for ERα between 24 M1 (red) and 3 M2 (blue) tumors. Pearson correlation is depicted from 0 (white) to 1 (dark green) for all panels. c Heatmaps for all factors with known M1/M2 subtype classification, depicting raw signal intensities at differential bound ERα sites between M1 and M2. d Genomic distribution for differentially ERα-bound sites by M1 and M2 subtypes. e Bar plots indicating top 5 sequence motifs enriched in M1 and M2 differentially ERα-bound sites. f Ingenuity Pathway Analysis of Upstream Regulators and their p-values identified in both ERα binding site associated and Johannson et al.⁴ gene expression based driven M1/M2 genes. g Ingenuity Pathway Analysis of Canonical Pathways and their p-values identified in both ERα binding site associated and Johannson et al.⁴ gene expression based driven M1/M2 genes