Fig. 5
Strong conservation of ERα and FOXA1 binding between genders. a Correlation plot of ERα bound regions across all biological systems. Top row indicates female tumors (salmon), male tumors (red), and female cell lines ZR751 (dark green), T47D (green), MCF7 (light green) and MCF7 xenograft (yellow) classification. Overlap ratio is depicted from 0 (white) to 1 (red). b Boxplots depicting all overlap values for ERα bound regions in male (left) and female (right) samples. c Correlation plot of FOXA1 bound regions across all biological systems. Top row indicates female tumors (salmon), male tumors (red), and cell lines ZR751 (dark green), T47D (green) and MCF7 (light green) classification. Overlap ratio is depicted from 0.3 (white) to 1 (red). d Boxplots depicting all overlap values for FOXA1 bound regions in male (left) and female (right) samples. e Average ERα read count profiles for male ERα binding sites (50% consensus, top panel) and female ERα binding sites (50% consensus, bottom panel) in male (left) and female (right) datasets. 75% confidence interval of read count profiles are indicated with shading. f Average FOXA1 read count profiles for male FOXA1 binding sites (50% consensus, top panel) and female FOXA1 binding sites (50% consensus, bottom panel) in male (left) and female (right) data sets. 75% confidence interval of average profiles are indicated with shading. g Scatter plot depicting Z-scores of significantly enriched motifs at ERα binding sites in male (y-axis) and female (x-axis) tumors. h Scatter plot depicting Z-scores of significantly enriched motifs at FOXA1 binding sites in male (y-axis) and female (x-axis) tumors. i Average ERα read count profiles of male (left) and female (right) tumors at the differential ERα binding sites (±5 kb from the peak center) that can discriminate female outcome (top—good outcome enriched; bottom—poor outcome enriched). Patients are grouped based on outcome where indicated color is used for each group. 75% confidence interval of average profiles are indicated with shading
