Table 1 Turnover rates for PglH mutants

From: Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase

PglH

Turnover rate (Hexa-LLO·PglH−1 s−1)

WT

2.00 ± 0.34

H118A

0.19 ± 0.03

T271A

0.34 ± 0.08

E267A

0.008 ± 0.001

E275A

0.006 ± 0.001

R191A

No activitya

K196A

No activitya

E18A

No activitya

K75A

0.19 ± 0.01

R72A

0.31 ± 0.01

K68A

0.69 ± 0.04

K75A, R72A

0.039 ± 0.005

R72A, K68A

0.15 ± 0.07

K75A, R72A, K68A

0.002 ± 0.001

  1. Activity measurements were performed as described in Fig. 1. Turnover rates represent the average of 3 separate measurements ± s.d.
  2. aNo activity: turnover rate could not be calculated because no product could be detected or its level was below the detection limit of the assay
Back to article page