Fig. 4
From: Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody
Interaction of TBEV virions with Fab fragments of neutralizing antibody 19/1786. a Cryo-EM micrograph of TBEV virions incubated with Fab fragments of 19/1786. Scale bar represents 100 nm. b Electron-density map of Fab-covered TBEV virion rainbow-colored according to distance from center of particle. The right half of the image represents a B-factor sharpened map. Electron densities corresponding to the Fab fragments are located close to the 3-fold and 5-fold symmetry axes of the virion. c Molecular surface of TBEV virion covered with Fab 19/1786 fragments low-pass filtered to 7 Å resolution. E-proteins are shown in red, green, and blue. Fab fragments are shown in magenta (heavy chain) and pink (light chain). Scale bars in b and c represent 10 nm. d Footprints of Fab 19/1786 on TBEV surface. Fab fragments bind to two of the E-proteins of the asymmetric unit. The Fabs interact with an additional E-protein from the neighboring asymmetric unit shown in faint colors. e The Fab 19/1786 binds to the domain III at an angle of 135° relative to the axis of the E-protein ectodomain. The E-protein domain I is shown in red, domain II in yellow, domain III in violet, and domain IV in blue. The heavy chain of the Fab fragment is shown in magenta and the light chain in pink. The leaflets of the viral membrane are represented by gray dashed lines
