Fig. 1 | Nature Communications

Fig. 1

From: Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches

Fig. 1The alternative text for this image may have been generated using AI.

Proposed pathway for breviscapine biosynthesis in E. breviscapus and construction of the apigenin-7-O-glucuronide producing platform strain. a Proposed pathway for breviscapine biosynthesis in E. breviscapus. Genes that have been reported are shown in blue and those which were identified in this study are shown in red. Abbreviations: apigenin-7-O-G, apigenin-7-O-glucuronide; 4CL, 4-coumaroyl-CoA ligase; C4H, cinnamate 4-hydroxylase; CHI, chalcone isomerase; CHS, chalcone synthase; FSII, flavone synthase II; F6H, flavone-6-hydroxylase; F7GAT, flavonoid-7-O-glucuronosyltransferase; PAL, phenylalanine ammonia lyase; UDPGDH, UDP-glucose dehydrogenase. b Construction of the apigenin-producing strain SC1. c HPLC analysis of the apigenin-7-O-glucuronide standard, the fermented products of the apigenin-7-O-glucuronide producing strain (SC1-Y33-EbF7GAT-EbUDPGDH) and the negative control strain (SC1-Y33). Y33 is the abbreviation of plasmid YCPlac33. d LC-MS analysis of apigenin-7-O-glucuronide in the fermented products of SC1-Y33-EbF7GAT-EbUDPGDH

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