Fig. 3 | Nature Communications

Fig. 3

From: Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches

Fig. 3The alternative text for this image may have been generated using AI.

P450 enzymes screening and functional identification. a Overview of the P450 gene screening method. The detailed method is described in Methods. b HPLC analysis of the apigenin-7-O-glucuronide standard, scutellarin standard, the fermented product of the scutellarin-producing strain (SC1-Y22-EbF6H-EbCPR&Y33-EbF7GAT-EbUDPGDH), and the negative control strain (SC1-Y22&Y33). Y22 and Y33 represent the plasmids Ycplac22 and YcplacY33. c MS analysis of scutellarin in the fermented product of the scutellarin-producing strain. d HPLC analysis of microsomal enzyme assays (containing EbF6H and EbCPR) incubated with apigenin in vitro. The microsomal fraction containing only EbCPR was used as negative control. e HPLC analysis of microsomal enzyme assays incubated with apigenin-7-O-glucuronide in vitro. The microsomal fraction containing only EbCPR was used as the negative control

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