Table 1 Disruption of predicted RNA structures attenuates IAV
Virus | NP-bound | MFE | Effect of mutations | Effects on virus | |||
|---|---|---|---|---|---|---|---|
Focus size | Lung titer | Genome packaging | |||||
TCID 50: HA ratio | Segment packaging | ||||||
NP22–68:A | -- | −8.7 | ↓–ΔG | ↓↓ | ↓ | ↓↓ | ↓↓ |
NP22–68:B | -- | −8.7 | No effect | = | = | = | = |
NP145–175 | = | −8.0 | ↓–ΔG | = | = | = | = |
NP456–490 | = | −3.2 | No effect | = | = | = | = |
NP584–608 | = | −0.0 | No effect | = | = | = | = |
NP1058–1081 | = | −0.0 | No effect | = | = | = | = |
NP1410–1495 | -- | −19.8 | Modified Pseudoknot | ↓↓ | ↓↓ | ↓↓ | ↓↓ |
PB2350–375 | = | −0.0 | No effect | = | N.D. | = | = |
PB21823–1944:A | -- | −33.0 | Modified Pseudoknot | ↓ | ↓↓ | ↓↓ | = |
PB21823–1944:B | -- | −33.0 | Modified Pseudoknot | ↓ | N.D. | ↓ | = |
PB21823–1944:C | -- | −33.0 | Modified Pseudoknot | ↓ | N.D. | = | = |
PB22213–2239 | = | −0.0 | No effect | = | N.D. | = | = |
PB1497–561:A | -- | −12.9 | ↓–ΔG | ↓ | ↓ | ↓↓ | ↓↓ |
PB1497–561:B | -- | −12.9 | No effect | = | N.D. | = | = |
PB11828–1858 | = | −3.8 | No effect | = | N.D. | = | = |
PB12032–2058 | = | −2.2 | No effect | = | N.D. | = | = |
NS23–86:A | -- | −10.0 | Modified Pseudoknot | ↓↓ | ↓ | ↓↓ | ↓↓ |
NS23–86:B | -- | −10.0 | No effect | = | N.D. | = | = |
