Fig. 3

2D and 3D quantification reveals a dramatic decrease in the proliferative capacity of αMHC-expressing cells. Quantification of clonal expansion in P2 βactin^CreER; R26^VT2/GK, Nkx2.5^CreER; R26^VT2/GK, and αMHC^CreER; R26^VT2/GK hearts labeled at E9.5 (a, b, e, f) and E12.5 (c, d, g, h) (two-sample Kolmogorov–Smirnov distribution test) *p < 0.05, **p < 0.01, ***p < 0.001. Two dimensional quantification of clonal expansion (a, c) and bar graphs highlighting the scarcity of large clones in αMHC^CreER; R26^VT2/GK hearts (b, d), respectively. Three-dimensional quantification of clone volumes in P2 hearts (e, g) and bar graphs depicting clones volumes in αMHC^CreER; R26^VT2/GK hearts (f, h). Green lines depict mean values. Dashed lines depict cutoff