Fig. 2
From: Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis
Unbiased clustering of fibroblasts by single-cell RNA-seq. a Hierarchical clustering is concordant with our surface protein gating. The first track indicates which donor the cell came from. The second track indicates which cell sorting gate the cell falls into, by surface protein expression. The heatmap indicates mRNA expression, scaled across cells to reveal variation between cells. Below the heatmap, the first track shows linear discriminant analysis (LDA) classification by mRNA expression of 968 genes. The last track shows posterior probabilities of LDA classification. b For the heatmap, we selected 23 genes with greatest 1% mean and greatest 1% variance in expression levels. c The number of cells in each subset as determined by surface protein levels (x-axis) and by LDA classification (y-axis) is consistent. RMSE, root mean squared error
