Fig. 5
From: Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis
Functional differences between fibroblast subsets. a Gene expression pattern of key effector molecules to predict the function of fibroblast subsets. b Gene set enrichment analysis with RNA-seq samples and MSigDB Hallmark Gene Signatures. Terms with <5% FDR are displayed. c The proportion of Ki67-positive cells in freshly isolated synovial fibroblast subsets. d Protein expression of MMP14 in the cell lysates of expanded fibroblast subsets in the presence of 1 ng/mL TNF-α. e The number of migrated and invaded cells in transwell matrix invasion assay. Fifty nanogram per mL of PDGFBB was used to promote migration and invasion. f The ratio of TNFSF11 (RANKL) and TNFRSF11B (OPG) in mRNA level in expanded fibroblast subsets. g Protein expression of TNFRSF11B in the supernatant of expanded fibroblast subsets. h Number of osteoclasts developed in the co-culture of osteoclast progenitors with fibroblast subsets in the presence of 20 ng/mL M-CSF and 5 ng/mL RANKL. i Protein expression of cytokines and MMPs in the supernatant of expanded fibroblast subsets in the presence of 1 ng/mL TNF-α. j The number of migrated monocytes in monocyte recruitment assay using supernatant of cultured fibroblast subsets. In bar graphs, blue, red and green color coding corresponds to the fibroblast subsets shown in a. White bar in j indicates culture media without fibroblasts. Bars show means and error bars show standard deviations. One-way ANOVA, Tukey's post hoc: *P < 0.05; ** P < 0.005
