Fig. 1
From: Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair
Dicer and Drosha are required for formation of 53BP1 foci after ionizing radiation (IR). a Representative immunofluorescence images visualizing IR-induced 53BP1 foci in A549 cells. 53BP1 in green channel, γH2A.X in red, DAPI-stained nuclei in blue. Scale bars, 10 µm. b Quantification of a. The number of foci per nucleus was counted using the FindFoci ImageJ plugin and plotted as individual data points (gray) and a violin plot. Data from 3 biological replicates, counting at least 60 cells per replicate. Red line represents the median in each condition. Statistical testing was performed using Dunn’s test with Bonferroni correction for multiple comparisons, ***p ≤ 0.001. c Representative western blots for a, b confirming knockdowns and induction of DNA damage
