Fig. 6
From: Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes
Design of the feedback controller. a Function and robustness of the controller decoupling in comparison to using the o-ribosome pool alone. The optimal controller parameters were perturbed by drawing random values (N = 1000) between ±50% of the original value. All parameters controlling o-rRNA and controller protein were allowed to vary. Inset, mRNA levels of RFP and GFP. Legend explanation: o-ribo, uncontrolled o-ribosome pool (i.e. when ω F = 0 mRNAs per min); Controller, optimal feedback controller; Robustness, action of the perturbed controllers. b Impact of varying the maximal transcription rate of the o-rRNA. c Impact of varying the strength of the RBS d Impact of varying the dissociation constant of the repressor. e Impact of varying the Hill function coefficient of the repressor
