Fig. 3
From: Ikaros family zinc finger 1 regulates dendritic cell development and function in humans
Dose-dependent reduction of IKZF1 protein and pDCs in patients receiving lenalidomide treatment. a Intracellular flow cytometric quantification (mean fluorescence intensity (MFI)) of IKZF1 protein levels in B cells (identified as SSClowLin−DR+CD2− cells) of a family G member carrying IKZF1 mutation, patient on lenalidomide treatment, a healthy donor and isotype control. b Intracellular IKZF1 level (MFI) in healthy donors (n = 5), patients tested at the end of a week off treatment with specified dose of lenalidomide (n = 16) (5, 10, 15 or 25 mg daily) and patients taking lenalidomide (Len) on the day of analysis (n = 3) (15 mg daily). Bars represent mean ± s.d. *p = 0.036 by two-tailed Mann–Whitney U-test versus healthy donor controls. c Summary of absolute B-cell counts in whole blood from healthy donors (n = 4) (Cont), patients tested at the end of a week off treatment with specified dose of lenalidomide (n = 16) (5 or 10, 15 or 25 mg daily) and patients taking lenalidomide on the day of analysis (n = 3) (15 mg daily). Bars represent mean ± s.d. *p = 0.006 Mann–Whitney U-test versus healthy donor controls. d IKZF1 protein levels (MFI) plotted against absolute plasmacytoid dendritic cell (pDC) counts in affected (n = 3) and unaffected (n = 1) members of family G (white or grey open circles, respectively), patients after a week off lenalidomide (n = 22) (black circles) or taking lenalidomide on the day of analysis (n = 3) (black diamond) or healthy donors (n = 4) (grey circles). Data from two patients taking pomalidomide (4 mg daily) are displayed (grey triangles). Black line represents linear regression analysis of lenalidomide-treated patients only. Inclusion of family G members and healthy controls in the linear regression analysis resulted in r2 = 0.6541, p < 0.0001. Inclusion of pomalidomide-treated patients resulted in r2 = 0.6419, p < 0.0001. Dashed line represents IKZF1 MFI = 680 dividing group R1 (IKZF1 MFI > 680) from group R2 (IKZF1 MFI < 680). e PB absolute monocyte and DC counts in healthy controls (C) compared to patients from group R1 and R2. The p-values from two-tailed Mann–Whitney U-test of R2 versus healthy donors
