Table 2 Variant calling metrics of Illumina PCR-free paired-end reads against the assemblies and Illumina PCR-free paired-end reads from Col-0 mapped against the TAIR10 reference assembly

From: High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell

Name

Round (#)

Total variants (#)

SNPs (#)

Insertions (#)

Deletions (#)

ONTcan

0

2,505,080

281,421

2,202,363

21,296

ONTcan

1

1,782,042

276,025

1,432,415

73,602

ONTcan

2

1,723,244

266,625

1,384,435

72,184

ONTcan

3

1,706,676

264,720

1,369,595

72,361

ONTcan

4

99,252

73,616

10,244

15,392

ONTmin

0

7,208,897

3,359,123

2,911,987

937,787

ONTmin

1

1,994,370

345,671

1,532,261

116,438

ONTmin

2

1,767,580

287,706

1,393,680

86,194

ONTmin

3

1,730,743

278,301

1,369,470

82,972

ONTmin

4

91,544

67,062

9026

15,456

PBfal

0

474,355

62,063

367,338

44,954

PBfal

1

39,433

26,486

6860

6087

PBfal

4

27,312

24,744

1010

1558

TAIR10

16,807

13,336

1770

1121

  1. Assembly types: Oxford Nanopore MinION, ONT; PacBio Sequel, PacBio; Miniasm, min; Falcon, fal; Canu, can; reference genome, TAIR10. Polishing rounds PBfal: 0 = raw assembly; 1, arrow 1x; 4, pilon 1x