Fig. 4
Classification and characterization of TAD boundaries according to insulation score (high sequencing depth = 80 million intrachromosomal read pairs). a Workflow of stratification of TAD boundaries by insulating score. b Heatmap representation of TAD boundary insulation strength across samples; hierarchical clustering correctly groups replicates and related cell types independent of enzyme biases or batch effects related to the lab that generated the Hi-C libraries (detailed information about all Hi-C data sets and their cell types is included in Supplementary Data 1). c TAD boundary strength is associated with CTCF levels. d Comparison of the association of “ratio” vs. “crane” insulation scores with CTCF levels. e Fraction of super-enhancer elements in the vicinity of boundaries of variable strength. f Comparison of the association of “ratio” vs. “crane” insulation scores with respect to proximity to super-enhancers. All statistical tests are paired two-sided Wilcoxon rank-sum tests between distributions defined across samples (each sample is a dot in the boxplots). The box in each boxplot represents the first (Q1) and third (Q3) quartiles and the ends of the whiskers are positioned 1.5*(Q3-Q1) away from the ends of the box.
