Fig. 2

BRE overexpression rescues Brca2^KO/KO cell viability without affecting BRCA2 function. a Ionizing radiation (IR)-induced RAD51 foci formation in Brca2^CKO/KO and Brca2^KO/KO; MSCV-BRE ES cells. RAD51 foci are in turquoise, γ-H2AX foci in magenta, and nuclei are stained with 4,6-diamidino-2-phenylindole (DAPI, blue). Merged images show co-localization of RAD51 and γ-H2AX foci (yellow foci). b Quantification of RAD51 foci shown in a. Twenty-five nuclei were counted for each genotype. Error bars represent the mean ± s.d. c Scatter plot showing DNA fiber analysis of the indicated cell lines. P values were calculated using Mann–Whitney test. d Methylene blue-stained plates showing the sensitivity of Brca2^CKO/KO and Brca2^KO/KO; MSCV-BRE ES cells to different doses of IR. e Quantification of the colonies shown in d. Colony numbers obtained in plates with no treatment (0 Gy) are considered as 100%. Average of three experiments is shown. Error bars represent s.d. values. f Representative metaphase spread showing chromosomal aberration (marked with arrows) in Brca2^CKO/KO and Brca2^KO/KO; MSCV-BRE ES cells. g Quantification of different aberrations as well as total aberrations in 50 randomly selected Brca2^CKO/KO and Brca2^KO/KO; MSCV-BRE metaphase spreads