Fig. 2
From: Combinatorial hydrogels with biochemical gradients for screening 3D cellular microenvironments
Effects of HAV and RGD gradients on transcription factor Sox9 expression. Maximum projection images and quantification across 10 regions of nuclear Sox9 fluorescence (aqua) for gradients of a, b RGD and c, d HAV peptides after 1 day. Representative maximum projection images of nuclei stained for Sox9 in e high or f low RGD regions and g high or h low HAV regions. Generally, higher nuclear Sox9 was observed with decreasing RGD and for increasing HAV. Error bars represent standard error around the mean (s.e.m.); n > 300 cells per zone, ***p < 0.001, *p < 0.05 compared to lowest peptide region (gray dashed line). Scale bars: a, c 1 mm, e–h 10 µm
