Fig. 3

eIF4B regulates translation of oncogenes. a Indicated eIF4B depleted stables were lysed and lysates were probed with the defined antibodies. Actin was used as loading control. b qRT-PCR analysis for expression of defined genes in indicated stable cells infected with shRNA against eIF4B or NT (scrambled). Results were normalized with NT infected corresponding cells and expressed as mean ± SD (n = 3). Statistical analysis was performed using Student’s t-test (unpaired two-tailed), *p < 0.05, **p < 0.01 vs. NT infected corresponding cells. c Exponentially growing eIF4B depleted stable SUDHL2 cells were cultured with MG132 (10 µM) for 2 h and cell lysates were probed with the defined antibodies. Actin was used as loading control. N: scrambled; 1: sheIF4B-1; 2: sheIF4B-2. d Total cell lysates from SUDHL6 were subjected to immunoprecipitation with eIF4B antibodies, followed by RNA isolation and qRT-PCR to detect the enrichment of the defined genes. Results were normalized total RNA from cell lysates and expressed relative percentage to mock sample enriched RNA. Values are expressed as mean ± SD and statistical analysis was performed using Student’s t-test, *p < 0.05, **p < 0.01, ^αp < 0.001 vs. corresponding mock samples. e Indicated eIF4B depleted stable cells were lysed and lysates were probed with the defined antibodies. Vinculin was used as loading control