Fig. 5

Functional outcome of mTORC1 inhibition by NR1. a Assessment of NR1 effect on cell size in Jurkat cells compared to Torin-1 and rapamycin. Jurkat cells were treated with NR1 over the indicated concentration range, Torin-1 (250 nM), or rapamycin (100 nM) for 48 h, and cell size was quantified as a measure of forward scatter using flow cytometry. Data are plotted as percent decrease in forward scatter. Error bars represent standard deviation of the mean of quadruplicates; graph represents data from one of three separate experiments. b Evaluation of NR1 impact on protein synthesis compared to Torin-1 and rapamycin as measured by the incorporation of ³⁵S-methionine into total protein. MCF-7 cells were treated with NR1 at the indicated concentration range, Torin-1 (250 nM), or rapamycin (100 nM) for 2.5 h and labeled with ³⁵S-methionine for 0.5 h. Error bars represent standard deviation of the mean of duplicates; graph represents data from one of two separate experiments