Table 3 Rates of scarless editing at the APRT locus using engineered microhomology
From: Microhomology-assisted scarless genome editing in human iPSCs
Parent clone | Genotype | Excision (%mChneg) | Samples analyzed | Normal allele | NHEJ (perfect) | Scarless excisiona | Fidelity (%)b | Silent ONLY | APRT*J & Silent |
|---|---|---|---|---|---|---|---|---|---|
052-2-2 | Het | 8.4 | 37 | 23 | 9 (5) | 5 | 35.7 | 1 | 4 |
052-2-13 | Het | 5.0 | 50 | 30 | 17 (8) | 3 | 15.0 | 1 | 2 |
052-2-21 | Het | 6.8 | 46 | 24 | 14 (11) | 8 | 36.4 | 4 | 4 |
052-2-11 | Homo | 3.4 | 45 | 0 | 40 (26) | 5 | 11.1 | 0 | 5 |
052-2-52 | Homo | 3.6 | 53 | 0 | 47 (30) | 6 | 11.3 | 1 | 5 |
052-2-61 | Homo | 2.9 | 46 | 0 | 38 (30) | 8 | 17.4 | 0 | 8 |
