Fig. 2 | Nature Communications

Fig. 2

From: Cellular imaging by targeted assembly of hot-spot SERS and photoacoustic nanoprobes using split-fluorescent protein scaffolds

Fig. 2The alternative text for this image may have been generated using AI.

Formation of AuNP clusters by the assembly of FP fragments. a Agarose gel electrophoresis of sGFP-AuNPs, M3-AuNPs and unpurified sGFP-AuNPs + M3-AuNPs mixture and typical TEM images of clusters assembled with 40/40 nm AuNPs (top), 40/20 nm AuNPs (middle), and 40/10 nm AuNPs (bottom). White rectangles indicate the position of cluster bands in gels. Scale bars: 20 nm. b TEM images of clusters formed by the assembly of M3-AuNPs with sGFP-AuNPs, sYFP-AuNPs, or sCFP-AuNPs. Scale bars: 200 nm. c Normalized AuNP cluster size distribution and fit by a power law distribution model. d Size distribution of n = 314 nanogaps formed by the assembly of FP fragments between AuNPs in clusters. The distribution is Gaussian and centered at 2.1 ± 0.5 nm (mean ± s.d.). Inset: expected orientation of complemented GFP at nanogaps. e TEM images of clusters formed by the assembly of sGFP-gold nanorods with different sizes of M3-AuNPs. Scale bars: 20 nm

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