Fig. 3
The physical interaction between DEP1 and OsMADS1. a SFLC assays. nLUC-tagged DEP1 (or dep1-1) was co-transformed into tobacco leaves along with either the cLUC-targeted OsMADS1 or cLUC-targeted OsMADS1lgy3. The interaction of the rice GA receptor GID1 and the rice DELLA protein SLR1 were used as positive control. b Co-immunoprecipitation assays of Flag-DEP1 and HA-OsMADS1, and Flag-dep1-1 and HA-OsMADS1. IB Immunoblot, IP immunoprecipitation. c The image shows a SFLC assay in which nLUC-tagged deleted and non-deleted versions of OsMADS1 were co-transformed into tobacco leaves along with cLUC-DEP1. d The effect of the OsMADS1-DEP1 interaction on OsMADS1-induced transactivation activity. Deleted and non-deleted versions of OsMADS1 were fused to the GAL4-binding domain (GAL4BD). The relative activity of firefly luciferase (LUC) under control of the GAL4-binding element UAS was measured. Renilla luciferase (REN) activity was used as reference and VP16 as the positive control. Data shown as mean ± s.e.m. (n = 6). Statistical analyses were performed by Duncan’s multiple range tests. The presence of the same lowercase letter denotes a non-significant difference between means (P > 0.05)
