Fig. 2
From: Deficiency of a triterpene pathway results in humidity-sensitive genic male sterility in rice
The absence of OsOSC12 results in male sterility. a OsOSC12 mutants induced by either EMS or sodium azide. Boxes and lines indicate the OsOSC12 exons and introns, respectively. TILLING was directed at exons 6–10 and 16–18 (blue box). Mutated nucleotides are marked in red, and the changed nucleotides and the corresponding amino acids are shown for each mutant. b Northern blot analysis of OsOSC12 transcription. c Western blot analysis of OsOSC12 expression. Negative controls as given in Fig. 1. d Self fertility of field-grown WT and mutant plants (30–39 °C, 40–70% RH), based on panicles. The data are presented as mean ± s.d. n = 15. e Germination (upper panel) and pollen tube elongation (lower panel) of WT and E157 pollen, imaged 1 h after pollination. Scale bar, 100 μm
