Fig. 4
From: Deficiency of a triterpene pathway results in humidity-sensitive genic male sterility in rice
Identification of pollen coat chemicals. TEM analysis of ultramicrotome (a) and cryo-ultramicrotome (b) sections of the WT and E157 pollen wall at S14. c Proposed mode of pollen coat formation during stages S9 to S12. Ba bacula, Cy cytoplasm, In intine, LTPs lipid transfer proteins, Lo locule, Ne nexine, Pc pollen coat (indicated by red arrows), PE primexine, Pla plasma membrane, Te tectum, U ubisch body, Vac vacuole. d Analysis of pollen coat lipids of the WT and E157 plants. The values indicate means ± s.d., n = 5, **P < 0.01, by Student’s t test. e Pollen dehydration rates when treated with pollen coat extracts and chemicals. E157-hexane: E157 pollen treated by hexane, WT-hexane: wild-type pollen treated by hexane, abbreviates of fraction and chemical names are listed in Supplementary Table 4. Times of pollen (n = 100–150) dehydration are presented in the form of mean ± s.d., n = 3. Scale bar, 0.5 μm
