Fig. 6
From: Deficiency of a triterpene pathway results in humidity-sensitive genic male sterility in rice
HGMS-based hybrid seed production. a Seed setting rate and hybrid rate of the crosses of E157 and S4928 plants, with the WT plants. The data are presented as mean ± s.d., n = 15 panicles for seed setting rate, n = 300 seeds for hybrid rate. b The variation in RH and temperature at the field site over the period of anthesis. Data were recorded at 1 h intervals by a detector suspended 10 cm above the canopy. c A simplified phylogeny of plant OSCs, based on their coding sequence. A maximum likelihood tree was constructed assuming the GTR + Γ + I model. The gray triangles indicate groups of OSCs derived from the same lineage. Sequences shown in bold indicate poaceatapetol synthase-like genes. d Northern blotting analysis of transcription in the anthers of four major cereal species. e Western blotting demonstrates the presence of OsOSC12-like proteins in the anthers of other cereal species. f The GC/MS profile of the triterpene alcohol fraction of extracts of barley, maize and wheat anthers. Standard, purified poaceatapetol; EIC 189, extracted ion chromatograms at m/z 189. The arrow indicates the poaceatapetol peak
