Table 1 Principal co-localisation index
From: Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics
Name | Type | Statistics | Pros | Cons | References |
|---|---|---|---|---|---|
Pearson Correlation Coefficient (PCC) | Correlation | Pixel scrambling or Analytics | Easy to use; Works with any type of signal (diffuse, spotty, filaments…) | Depends on microscope resolution; Does not apply to localisation-based microscopy; Hardly interpretable in terms of spots’ coupling | |
Cross-Correlation Spectroscopy | Correlation | Pixel scrambling or Analytics | Easily interpretable; Works with any type of signal | Depends on microscope resolution; Does not apply to localisation-based microscopy; Sensitive to local variations of intensity | |
DeBias | Correlation | Pixel scrambling | Allows to separate global bias from local interactions; Works with any type of signal | Depends on microscope resolution; Does not apply to localisation-based microscopy | |
Manders Overlap Coefficient (MOC) | Overlap | Spots’ randomisation or Analytics (for ideal disk-shape spots68) | Easily interpretable; Works with any type of signal | Depends on microscope resolution; Does not apply to localisation-based microscopy; Randomisation can be computationally expensive | |
Thresholded Overlap (TO) | Overlap | Spots’ randomisation or Analytics (for ideal disk-shape spots69) | Same as MOC, Possible selection of individual coupled spots with thresholded-overlap | Same as MOC, one more tunable parameter (threshold) | |
Mass-centre inside Mask (MM) | Distance-based (1st order, measures the density of (red) points in (green) masks) | Analytics | Same as TO | Depends on microscope resolution; Does not apply to localisation-based microscopy | |
Distance to Nearest-Neighbour (NN) | Distance-based (2nd order, measures the distance between neighbours) | localisations’ randomisation | Does not depend on microscope resolution; Apply to localisation-based microscope | Global index (interaction strength15 or False Discovery Rate10) hardly interpretable in terms of coupling; Apply to spotty objects only | |
Co-clustering of localisations | Correlation between localisations’ clusters | localisations’ randomisation | Apply to localisation-based microscopy | Hardly interpretable in terms of coupling; Not robust to mean coupling distance >0 | |
SODA | Distance-based (2nd order) | Analytics | Same as NN; Statistical mapping of individual couples of objects (spots, localisations) | Apply to spotty objects only | This study |
