Fig. 2
From: A dual mechanism promotes switching of the Stormorken STIM1 R304W mutant into the activated state
STIM1 R304W impact of hydrophobicity. All experiments performed with HEK 293 cells. a Patch clamp recordings of YFP-Orai1 + CFP-STIM1 R304X hydrophobic mutants (R304X = F (blue), W (red), L (black), V (green)). b Patch clamp recordings of YFP-Orai1 + CFP-STIM1 R304X hydrophilic mutants (R304X = K (orange), E (cyan), wt (violet)). c Bar diagram of patch clamp recordings showing initial current densities of HEK 293 cells expressing YFP-Orai1 + CFP-STIM1 R304X (R304X = W (red), L (black), F (blue), V (green), H (magenta), Q (olive), A (marine blue), E (cyan) K (orange), wt (violet)). *Significant difference (p < 0.05) to wt. d FRET measurements of HEK 293 cells expressing conformational sensor YFP-OASF-CFP R304X mutants. The color code corresponds to c. *Significant difference (p < 0.05) to wt. Error bars are defined as SEM. Statistics are Student’s t‐test
