Fig. 4
From: A dual mechanism promotes switching of the Stormorken STIM1 R304W mutant into the activated state
Constitutive activity of STIM1 R304W independent of Q314 E318. a Cartoon representing a crystallized STIM1 CC1 dimer21, 40 with highlighted interaction between R304 or R304W, respectively, with – Q314 and −E31821. b Bar diagram of FRET measurements of HEK 293 cells expressing conformational sensor YFP-OASF-CFP wt (black) and mutants (R304W (red), Q314W (blue), Q314K (magenta), E318W (violet), E318K (green)). *Significant difference (p < 0.05) to wt. n-number in brackets. c Patch clamp recordings of HEK 293 cells co-expressing YFP-Orai1 + CFP-STIM1 wt (black) or mutants (color code corresponds to b). Error bars are defined as SEM. Statistics are Student’s t‐test
