Fig. 5
From: A dual mechanism promotes switching of the Stormorken STIM1 R304W mutant into the activated state
STIM1 CC1 R304W does not perturb the CC1–CC3 fragment interaction. All experiments performed with HEK 293 cells. a Bar diagram of FRET analysis (FIRE system) showing CTMG-CC3 + YTMG-CC1 wt (black) and mutants (red, R304W; blue, L251S), respectively. Control (gray) represents YTMG-CC1 co-expressed with empty CTMG. *Significant difference (p < 0.05) to (CC1 + CC3). n-number in brackets. b YFP-OASF-CFP conformational sensor FRET analysis with wt (black), R304W (red), L251S (blue), L251S + R304W (red + blue), and R304W + R426L (red + orange). *Significant difference (p < 0.05) to wt. n-number in brackets. c Patch clamp recordings of HEK cells overexpressing YFP-Orai1 + CFP-STIM1 wt (black) or mutants (R304W; L251S; double L251S + R304W; and double R304W + R426L). Error bars are defined as SEM. Statistics are Student’s t‐test
