Fig. 6
From: A dual mechanism promotes switching of the Stormorken STIM1 R304W mutant into the activated state
CC1 R304W increases CC1-CC1 homomerization. All experiments performed with HEK 293 cells. a Schematic representation of STIM1. b Bar diagram of FRET analysis (FIRE system) showing homomerization of CC1 wt or mutants (R304W, L251S, double L251S + R304W, R304K). Control (gray) represents YTMG-CC1 co-expressed with empty CTMG. *Significant difference (p < 0.05) to CC1 (black). c FRET analysis (FIRE system) of homomerization experiments of CC1α1α2 wt or mutants (R304W, L251S, double L251S + R304W, R304K) (*significant difference (p < 0.05) to CC1α1α2 (black)); CC1α2α3 wt and R304W, respectively. n-number in brackets. Error bars are defined as SEM. Statistics are Student’s t‐test
