Fig. 4
From: Absence of warmth permits epigenetic memory of winter in Arabidopsis
VIN3 expression is regulated in a circadian manner. a, b VIN3 spliced and unspliced expression over 12 h. Plants grown in 20 °C night, 22 °C day 16 h photoperiod for 1 week and transferred to vernalising 8 h photoperiod with constant or fluctuating 14.2 or 8 °C profiles (as shown in Fig. 2a) for 4 weeks. The dark grey background indicates night-time. Error bars are s.e.m., n = 2–3, average > 2.7. c−f, VIN3 and various clock gene mRNA levels sampled on consecutive days at 8 °C after 4 weeks constant 8 °C, 8 h photoperiod (purple). On the first day of sampling the plants were transferred to constant light conditions (blue). The lighter grey background indicates subjective night. RNA levels normalised to UBC, PP2A. n = 1–3, average > 2.1. g Summary of VIN3 regulation showing the slow regulator (B), the new component (A) found to be necessary from modelling which gives the absence of warm regulation and the circadian clock, which could itself also be used as a temperature input
