Fig. 7
Upstream transcription factor 1 (USF1) transcriptionally regulates expression of INSM2 and uc.372. a Identification of usf1 elevated in the liver of 8-week-old male db/db mice (n = 5). b Identification of usf1 elevated in the liver of 10-week-HFD-fed mice (n = 5). c Expression of USF1 in the HepG2 cells treated with 300 μM O/P mixture for 48 h (n = 3). d Expression levels of USF1, uc.372, INSM2, and Nox2 in the HepG2 cells infected with Ad-USF1 or Ad-NC for 48 h (n = 3). e Protein levels of ACC, FAS, SCD1, and CD36 in the HepG2 cells infected with Ad-USF1 or Ad-NC for 48 h (representative blots from three similar experiments) (n = 3). f Real-time PCR analysis of USF1, uc.372, INSM2, and Nox2 in the HepG2 cells transfected with specific siRNAs targeting USF1 or NC for 48 h (n = 3). g Western blot analysis of ACC, FAS, SCD1, and CD36 expression in the HepG2 cells transfected with specific siRNAs targeting USF1 (representative blots from three similar experiments) (n = 3). h The mRNA level of uc.372 in the HepG2 cells infected with Ad-USF1 in the presence or absence of siINSM2 or NC for 48 h (n = 3). i The mRNA levels of USF1 and uc.372 in the HepG2 cells infected with Ad-USF1 and Ad-uc.372i for 48 h (n = 3). j The mRNA levels of ACC, FAS, SCD1, and CD36 in the HepG2 cells infected with Ad-USF1 and Ad-uc.372i or Ad-NC for 48 h (n = 3). Data are mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group. (a, b, c, d, e, g, i Student’s t-test; f, h, j analysis of variance (ANOVA))
