Fig. 3

Hematopoietic expression of ACKR2 increases monocyte and neutrophil mobilization. Absolute number of inflammatory monocytes (a) and neutrophils (b) in the blood of C57BL/6J WT or Ackr2^−/− mice 1 h after i.p. injection of CCL3L1 or vehicle (n = 11 for WT/PBS and 8 for Ackr2^−/−/PBS, 12 for WT/CCL3L1, and 10 for Ackr2^−/−/CCL3L1 for monocytes; n = 8 for both WT/PBS and Ackr2^−/−/PBS, 7 for WT/CCL3L1, and 5 for Ackr2^−/−/CCL3L1 for neutrophils). Percentage of monocytes (c) and neutrophils (d) in the BM of the indicated mice 1 h after CCL3L1 or vehicle i.p. injection (n = 19 for WT/PBS and 15 for Ackr2^−/−/PBS, 4 for both WT/CCL3L1, and Ackr2^−/−/CCL3L1 for monocytes; n = 12 for both WT/PBS and Ackr2^−/−/PBS, 7 for both WT/CCL3L1, and Ackr2^−/−/CCL3L1 for neutrophils). Absolute number of inflammatory monocytes (e) and neutrophils (f) in WT and Ackr2^−/− mice reconstituted with either WT (white columns) or Ackr2^−/− BM (black columns) after i.p. injection of CCL3L1 (n = 3 for both WT and Ackr2^−/− mice). Data are represented as mean (SD). p value was generated using the unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001