Fig. 4

Ackr2^−/− neutrophils are responsible for metastasis protection. a Metastatic ratio of C57BL/6J WT and Ackr2^−/− mice 10 days after i.v. injection of B16F10 cells (n = 14 for WT and 8 for Ackr2^−/−, sum of two independent experiments). b Metastatic ratio of C57BL/6J WT and Ackr2^−/− mice, treated with isotype IgG or with α-Ly6G, 10 days after i.v. injection of B16F10 cells (n = 6 for WT/isotype, and 5 for WT/α-Ly6G, Ackr2^−/−/isotype, and Ackr2^−/−/α-Ly6G). c Metastatic ratio of Balb/c WT and Ackr2^−/− mice, treated with isotype IgG or α-Ly6G antibody, 28 days after orthotopic injection of 4T1 cells (n = 3 for WT/isotype, WT/α-Ly6G, and Ackr2^−/−/α-Ly6G, and 5 for Ackr2^−/−/isotype). d Metastatic ratio in WT mice 10 days after i.v. injection of B16F10 cells and adoptive transfer of WT (white dots) or Ackr2^−/− neutrophils (black dots) or PBS (gray dots). Representative images of excised lungs are shown on the left (n = 5 for PBS, 9 for WT neutrophils, and 8 for Ackr2^−/− neutrophils). Data are represented as mean (SD). p value was generated using the unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001