Fig. 2
Vangl2 deletion in the uterine epithelium contribute to aberrant implantation and decidualization. a Day 5 implantation sites (blue bands) in Vangl2f/f and Vangl2f/fLtfCre/+ females and arrows indicate weak blue bands. b Histology of day 5 implantation sites in Vangl2f/f and Vangl2f/fLtfCre/+ mice. * embryo. Scale bar: 200 μm. c In situ hybridization of Ptgs2 and Bmp2 in Vangl2f/f and Vangl2f/fLtfCre/+ implantation sites on day 5. Scale bar: 200 μm. Arrowheads indicate the location of embryos. d 3D reconstruction of Scrib and Phalloidin localization at the apical surface of the epithelium by IF in Vangl2f/f and Vangl2f/fLtfCre/+ uterine luminal epithelium (LE) on day 4. Scale bar: 10 μm. e Cell shape analysis of uterine epithelial cells using SEGGA software. f, g Quantification of cell eccentricity and the long-to-short axis ratio in Vangl2f/f and Vangl2f/fLtfCre epithelial cells (Mean ± s.e.m. is derived from the indicated number of samples and analyzed by Student’s t-test, ***p < 0.001). h IF of Caspase3 and CK8 in day 6 implantation sites of Vangl2f/f and Vangl2f/fLtfCre/+ mice. Scale bar: 200 μm. i IF of CDH1 and CTNNB1 in day 6 implantation sites of Vangl2f/f and Vangl2f/fLtfCre/+ mice. Scale bar: 200 μm. All images are representative of three independent experiments
