Fig. 4

3D visualization of day 5 implantation sites in Vangl2f/f mice stained with CK8 amd FOXA2. a–f Original double immunostaining with CK8 and FOXA2 and 3D rendering pictures. CK8 markes both luminal epithelial and glandular epithelial cells, while FOXA2 only marks glands. Representative images of three independent experiments, images were generated by Nikon A1R Multiphoton Microscope; LWD 16× water objective with 3 μm Z-stack. * location of embryos. Scale bar: 400 μm