Fig. 5 | Nature Communications

Fig. 5

From: Tridimensional visualization reveals direct communication between the embryo and glands critical for implantation

Fig. 5The alternative text for this image may have been generated using AI.

3D visualization of day 6 implantation sites in Vangl2f/f and Vangl2f/fLtfCre/+ mice. a–c 3D images of CDH1, segmented and 3D rendered images of Vangl2f/f day 6 implantation sites generated by a light sheet microscopy. Scale bar: 200 μm. d–i 3D immunostaining with CDH1, segmented and 3D rendered images, respectively, in Vangl2f/f and Vangl2f/fLtfCre/+ mice generated by two-photon microscopy. Images are generated by Nikon A1R Multiphoton Microscope with a LWD 16× water objective with 3 µm Z-stack. Note: aberrant crypt size and evagination in Vangl2f/fLtfCre/+ mice with derailed extension and display of glands. * location of embryos. Scale bar: 300 μm. All images are representative of three independent experiments

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