Fig. 6 | Nature Communications

Fig. 6

From: Tridimensional visualization reveals direct communication between the embryo and glands critical for implantation

Fig. 6The alternative text for this image may have been generated using AI.

Implantation-competent blastocysts are required for crypt formation. a–f IF of E-Cad (CDH1), segmented and 3D rendering, respectively, in pseudopregnant days 4 and 5 uteri. psp pseudopregnancy. Scale bar: 200 μm. g–n, Original images of tdTomato reporter, segmented glands, and uterine lumen, 3D rendering and sectional view of implantation sites at 24 h after terminating delayed implantation by an injection of estrogen in P4-primed Rosa26tdTomatoLtfCre/+ mice. Asterisks indicate locations of embryos in delayed and activated uterus. Scale bar: 300 μm. Sectional views (j, n) are represented images (g, k). Images are generated by Nikon A1R Multiphoton Microscope with a LWD 16× water objective with 3 µm Z-stack. All images are representative of three independent experiments

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