Fig. 4
Microarray analyses of PCs and BMSCs in response to fracture. a Experimental design for microarray analyses of PCs and BMSCs isolated from wild-type un-injured tibias (d0) and from tibias 3 days post fracture (d3) (n = 4 per group). b Hierarchical clustering of biological replicates. c, d GSEA analyses of PCd0 vs. BMSCd0 and PCd3 vs. BMSCd3, respectively. PCs are enriched in stem cell, developmental, skeletal, and extracellular matrix gene sets (red) compared to BMSCs at both d0 and d3 (blue). e Number of differentially expressed probes in PCs and BMSCs in response to fracture. f Venn diagram showing the intersection of PCd3 vs. PCd0 and PCd3 vs. BMSCd3 representing the periosteum response to injury (PRI). g GSEA analysis of PRI genes. Red, blue, and gray boxes correspond to significant, interesting, and non-useful functions, respectively. Five significant functions are identified “response to external stimulus, “regulation of external stimulus”, “extracellular space”, “matrisome”, and “stem cell” (red). h The GSEA significantly enriched GO categories “response to external stimulus” and “regulation of external stimulus” were merged into “external stimulus” and compared by Venn to the “extracellular space” and “matrisome” GO categories resulting in a list of 9 common genes. i Venn diagram shows the intersection of PRI and Postn-linked genes resulting in a list of 6 genes (Complete list of 93 Postn linked genes in Supplementary Table 3)
