Fig. 2

CXCL12-CXCR4 axis is associated with basopenia in SLE. a Representative FACS analysis of CXCR4 levels on blood basophils as in Fig. 1b. b CXCR4 levels on blood basophils as in Fig. 1a (n = 95/32/24/ 70, respectively). c Representative images of CXCR4 (green) and FcεRIα (red) expressions by CT or active SLE patient basophil captured by imaging flow cytometry (scale bar = 5 µm). d Total CXCR4 expression and e CXCR4 externalization score in basophils from CT (n = 3) and active SLE patients (n = 4) as in (b) (20 basophils per individual). f CXCR4 RNA transcript expressions in basophils as in Fig. 1d. g CXCL12 levels in plasma from patients as in Fig. 1a (n = 78/45/38/ 87, respectively). h Basophils per µL of blood in patients with active SLE grouped by low (n = 59) or high (n = 22) CXCL12 plasma levels as defined in Fig. 1h. b, d, e, g, h Data are presented as median and interquartile ranges with whiskers representing 5–95 percentiles and the mean presented as a “+” symbol. Statistical analyses were by Mann−Whitney tests. f Data are presented as mean ± s.e.m. Statistical analyses were by unpaired Student t tests. b, d−h NS: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Comparison to control group is shown above each bar and to the corresponding bars when indicated. A.U. arbitrary units