Fig. 10
From: Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
Behavioral function of the APL neuron. a Silencing the APL neuron impairs odor-fructose association. Shown are the expression pattern of the split-Gal4 strain covering the APL neuron and a schematic of its innervation of the mushroom body (top left), associative performance indices for odor-fructose reward associative memory (top right), and a schematic of the behavioral task. Experimental larvae are heterozygous for the split-Gal4 driver and for UAS-Kir2.1::GFP (APL block). Control larvae are heterozygous for only the UAS-Kir2.1::GFP effector (effector Ctrl), or for only the split-Gal4 driver (driver Ctrl). Other details as in Fig. 3. The preference scores underlying the associative performance indices can be found in Supplementary Fig. 6. b Silencing the APL neuron does not impair innate odor preference; other details as in a. c Silencing the APL neuron does not impair innate fructose preference; other details as in a. d Optogenetic activation of the APL neuron during training abolishes odor-fructose association. Experimental larvae are heterozygous for the split-Gal4 driver as well as UAS-ChR2-XXL (APL activation). Control larvae are heterozygous for only the UAS-ChR2-XXL effector (effector Ctrl), or for only the split-Gal4 drivers (driver Ctrl). Other details as in Figs. 3 and 5a–d. The preference scores underlying the associative performance indices can be found in Supplementary Fig. 18. The bottom of the panel shows a schematic of the behavioral task. Blue shading indicates optogenetic stimulation. Other details as in Fig. 3. e As in d, showing that activation of the APL neuron during only the test reduces odor-fructose association scores. f, g As in b, c, showing that activation of the APL neuron in experimentally naïve animals affects neither odor preference nor fructose preference
