Fig. 3
From: Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila
Requirement of shaft and lower-toe MBE neurons for odor-fructose reward association, odor preference, and fructose preference. a–d Expression pattern of the indicated split-Gal4 strain and schematic overview of the covered MBE (leftmost column), associative performance indices for odor-fructose reward associative memory (second column), and preference scores of experimentally naïve larvae for the odor (third column) as well as for fructose (rightmost column). Experimental larvae are heterozygous for the indicated split-Gal4 drivers and for UAS-Kir2.1::GFP, leading to silencing of the respective MBE (MBE block). Control larvae are heterozygous for only the UAS-Kir2.1::GFP effector (effector Ctrl), or for only the split-Gal4 drivers (driver Ctrl). Box plots show the median as the middle line, and 25/75% and 10/90% quantiles as box boundaries and whiskers, respectively. Sample sizes are indicated within the figure. At plain horizontal lines * refers to P < 0.05/2 and ns to P > 0.05/2 in Mann–Whitney U-tests; at horizontal lines with arrowheads, ns refers to P > 0.05 in Kruskal–Wallis tests. Significant phenotypes upon silencing a given MBE are marked by red frames. The preference scores underlying the associative performance indices in the leftmost panels can be found in Supplementary Fig. 6. Expression patterns of split-Gal4 drivers covering the respective MBE are visible based on anti-GFP staining (black); the central nervous system is visible based on background fluorescence (gray). e From left to right the panel shows schematics of the odor-fructose reward association task, and of the odor preference or fructose preference tasks. The orange cloud indicates n-amylacetate as the odor, the green circle indicates the fructose reward. In half of the cases the sequence of training trials was as indicated in the leftmost display, while for the other half it was reversed (not shown)
