Fig. 3

Sc polarity is observed in tumour cells from patients. a Representative images of tumour cells expressing ezrin-GFP (ezrin) stained with phalloidin and DAPI or TO-PRO3. Quantifications of polarisation in suspension are shown in Supplementary Table 1. b Cells from pleural effusion (PE) of a pancreatic carcinoma (PC) and from ascitic effusion (AE) of a breast cancer (MC mammary carcinoma) patient stained for ezrin, EpCAM, CD45 and DAPI. Control stainings for PE(PC) are shown in Supplementary Fig. 3a. Additional PE cells from MC patients are shown in Supplementary Fig. 2. c Circulating tumour cells (CTC) from blood of two MC patients stained for cytokeratin (CK), ezrin and DAPI. Control stainings for CTC(MC) 2 are shown in Supplementary Fig. 3b. Further CTCs are shown in Supplementary Fig. 2. Images of CTCs during Cell Search isolation and confirmation of CTC identity by single-cell array comparative hybridisation (aCGH) are shown in Supplementary Figs. 4 and 5. d Single, disseminated tumour cells from lymph nodes of melanoma (mel-DTC, left) or lung cancer (LC-DTC) patients stained for gp100 or EpCAM, ezrin and DAPI. Scale bars: 10 µm. Controls are shown in Supplementary Fig. 3c. e Paraffin samples of established human primary (left) or metastatic (right) melanoma stained for ezrin, melan A and DAPI. Scale bars: 10 µm. Arrowheads indicate sc poles