Fig. 3
From: Host defense against oral microbiota by bone-damaging T cells
A crucial role for exFoxp3TH17 cells in the bone destruction during oral infection. a Frequency and number of exFoxp3TH17 cells in the periodontal tissues (Gum) and the cervical lymph nodes (CLNs) in control or periodontitis-induced mice 7 days after the ligature placement (n = 3). Representative data of more than three independent experiments is presented. PD: periodontitis. b Effects of adoptive transfer of TH17 cells (n = 8) or exFoxp3TH17 cells (n = 7) on periodontitis-induced bone loss compared to the saline group (n = 6). The data were pooled from more than three independent experiments. The upper red dotted line indicates the cementoenamel junction and the lower red dotted line indicates the alveolar bone crest in the left panel. All data are shown as the mean ± s.e.m. c Quantitative RT-PCR analysis of Rorc, Il17a, Il17f, and Tnfsf11 transcripts in TH17 cells or exFoxp3TH17 cells (n = 4). The data were pooled from two independent experiments. d FACS profiles of RANKL expression in TH17 cells or exFoxp3TH17 cells. Representative data of more than three independent experiments is shown. Statistical analyses were performed using Student’s t-test (a, c), ANOVA with Tukey’s multiple-comparison test (b). *P < 0.05; **P < 0.01; ***P < 0.005
