Fig. 5
From: Host defense against oral microbiota by bone-damaging T cells
IL-6 facilitates the generation of exFoxp3TH17 cells during periodontal infection. a Frequency and number of exFoxp3TH17 cells in periodontal tissues (Gum) and cervical lymph nodes (CLNs) in periodontitis-induced mice treated with saline or anti-IL-6R antibody (Ab) 7 days after the ligature placement (n = 3). Two milligrams of anti-IL-6R Ab (MR16-1) were injected intraperitoneally after 2 days of the ligature placement. Representative data of two independent experiments is shown. b Periodontitis-induced bone loss in control mice or anti-IL-6R Ab-treated mice (n = 5), pooled from two independent experiments. c Micro-CT analysis of periodontitis-induced bone loss in wild-type mice (n = 4) or Il6−/− mice (n = 5). The upper red dotted line indicates the cementoenamel junction and the lower red dotted line indicates the alveolar bone crest. d In situ hybridization of Il6 mRNA in periodontitis-induced wild-type mice 3 days after the ligature placement. Representative data of more than three independent experiments is shown. H&E, haematoxylin and eosin stain; E, epithelium; T, tooth; AB, alveolar bone. All data are shown as the mean ± s.e.m. Statistical analyses were performed using Student’s t-test. *P < 0.05; ***P < 0.005
