Fig. 8

Keratinocyte-specific depletion of miR-21 impairs macrophage conversion. a FSP1 (cyan) immunostaining and macrophages (GFP⁺, green) colocalization in d5 wound tissue from LysM^CreRosa^mT/mG mice where antago-miR-21 or scrambled oligo were topically delivered to the wounds, scale bar = 50 µm, scale bar of inset = 10 µm. b FSP1 intensity in scrambled and antago-miR-21 delivered mice, n = 5, Student’s t test p < 0.05. c q-RT-PCR analyisis of COL1A1 expression in LysM^CreRosa^mT/mG mice d5 wound tissues scrambled and antago-miR-21, n = 5, Student’s t test p < 0.05. d Macrophage (F4/80, red) and fibroblast (FSP1, green) immunostaining of d5 wound tissue from wt and miR-21^fl/flK14^Cre mice, n = 5, Student’s t test p < 0.05, scale bar = 10 µm. e FSP1 intensity analysis in wt(miR-21^fl/fl) and miR-21^fl/fl K14^Cre mice, n = 5, Student’s t test p < 0.05. f F4/80 (macrophage, red) and FSP1 (fibroblast, green) immunostained wound macrophages isolated from miR-21^fl/flLysM^Cremice. Rescue of FSP1 expression (green) with nanoparticles mediated miR-21 (magenta) delivery, scale bar = 10 µm. g FSP1 intensity in wound macrophages delivered with miR-21 compared to control mimic, n = 5, Student’s t test p < 0.05