Fig. 6

Cellular-level population light responses of RGCs in NW arrays-interfaced blind mouse retinas. a Expression of GCaMP6 in the whole-mount retina. Turquoise color represents cell bodies of RGCs that expressed GCaMP6, and magenta color represents Brn3a-labeled RGCs. Arrowhead with long tail: cells co-stained with Brn3a and GCaMP6. Arrowhead with short tail: cells stained with GCaMP6. b The cross section of the retina. INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. c Snapshot image of GCaMP6 fluorescence when no light stimulation was presented. Magenta and blue circles outline 52 light responsive and 66 non-responsive RGCs, respectively. Inset image shows the same snapshot image with no outlines of the RGCs. d All the 52 light responsive RGCs in c. Purple shade represents the presence of near UV light. e Three examples of non-responsive RGCs in c. f Change of fluorescence (ΔF/F₀) for all the responsive RGCs in c. Gray lines represent ΔF/F₀ curves for individual cells. Red line represents average ΔF/F₀ for all the RGCs. Purple lines represent the presence of near UV light. Scale bars: 20 μm (a, b), 50 μm (c)