Fig. 4
From: Reconstruction of complex single-cell trajectories using CellRouter
Multi-lineage hematopoietic stem cell differentiation. a Three-dimensional diffusion components analysis with subpopulation structure identified by CellRouter. b Identification of lineages based on expression of known marker genes with Procr expressed in HSCs and Gata1, Ccl3, and Ctsg expressed in the erythroid, lymphoid, and granulocyte-macrophage lineages, respectively. c Gene ontology analysis using genes dynamically upregulated in selected differentiation trajectories from HSCs to the erythrocytes (subpopulation 8), granulocyte/macrophage progenitors (subpopulation 11), and lymphoid progenitors (subpopulation 4). d Predicted regulators of transitions to the erythroid lineage (subpopulation 8). e Predicted transcriptional regulators of lymphoid development from HSCs (left panel) and their pseudo-temporal regulation (right panel). f Clustering of gene expression trends during lymphoid development from HSCs into five expression patterns, with a summary of the patterns in each cluster shown in the bottom right panel. g Representative genes in transcriptional clusters from f and the Jaccard similarity matrix of the lymphoid trajectory demonstrating the gradual divergence of single-cell transcriptomes during differentiation (bottom right panel). Accession code: GSE81682
