Fig. 2

Identification of MSD1 gene in sorghum. a Workflow of bulk segregant analysis combined with whole-genome sequencing, used to identify the causal gene in an msd1-1 F₂ population. b Structure of MSD1 (Sb07g021140), depicting the CDS of 774 nucleotides and the TCP domain. The table below shows alleles of msd1 identified by sequencing of 15 msd mutants. c Co-segregation of the msd1-1 mutation in Sb07g021140 with the msd phenotype was analyzed in an F₂ population derived from a cross of RTx437×msd1-1. Among the 48 individual F₂ plants, 10 with homozygous mutations (AA) had msd panicles, whereas the rest (11 homozygous WT [GG] and 27 heterozygous [GA]) had WT panicles. The phenotype distribution for this population fit a 3:1 WT:msd ratio, with 100% co-segregation of the mutant genotype with the phenotype