Fig. 3

MSD1 expression analysis in wild type. a Relative expression of MSD1 from qRT-PCR in various tissue types, including leaf, root, stem, petiole, SAM, and inflorescence (stages 1+2, 3, and 4) of PS, SS (stage 4), PS (stage 5), and SS (stage 5). The bars represent mean ± SD of three biological replicates. b–k In situ hybridization of MSD1 during inflorescence development in WT: b Spikelet meristem in stage 2, labeled with sense probe as a negative control. c Longitudinal view of shoot apical meristem. d Spikelet meristem near the top of meristem in stage 2, labeled with antisense probe. e Spikelet meristem at the bottom of meristem in stage 2. f–h Longitudinal section of floral meristem in early stage 3. g and h show magnifications of the floral meristem. i–k Transverse section of floral meristem in early stage 3. Magnifications of the floral meristem at early stage 3 in i are shown in j and k. l Cellular localization of MSD1: confocal images showing MSD1–green fluorescent protein (GFP) localization in tobacco transient assay. Top left: Nuclear Localization Sequence (NLS)-red fluorescent protein (RFP) (control); top right: MSD1-GFP; bottom left: differential interference contrast (DIC) image; bottom right: merged image of NLS-RFP and MSD1-GFP. b–k Bar: 100 μm. I Bar: 50 μm